The writer should has an experiense in Bioinformatics and genomics function. The aim of this review is to
review successful transformation methods such as natural and electroporation transformation of C. jejuni
and relate these appropriate methods to design a Campylobacter hepaticus transformation strategy. You
should review the difference and similarities between C. hepaticus and C. jejuni’s genomes and growth
characteristics then suggest the transformation strategy for C. hepaticus.
Below are several paper for you to start with, then you do more search for the review.
Davis, L., Young, K., & DiRita, V. (2008). Genetic Manipulation of Campylobacter jejuni. Current Protocols
in Microbiology, CHAPTER 8, Unit-8A.2.17. https://doi.org/10.1002/9780471729259.mc08a02s10
Davis, L., & DiRita, V. (2008). Growth and laboratory maintenance of Campylobacter jejuni. Current
Protocols in Microbiology, Chapter 8, Unit 8A.1.1-8A.1.7.
https://doi.org/10.1002/9780471729259.mc08a01s10
Wiesner, R. S., Hendrixson, D. R., & DiRita, V. J. (2003). Natural Transformation of Campylobacter jejuni
Requires Components of a Type II Secretion System. Journal of Bacteriology, 185(18), 5408–5418.
https://doi.org/10.1128/JB.185.18.5408-5418.2003
Wang, Y., & Taylor, D. E. (1990). Natural transformation in Campylobacter species. Journal of Bacteriology,
172(2), 949–955. https://doi.org/10.1128/JB.172.2.949-955.1990
Wassenaar, T. M., Fry, B. N., & van der Zeijst, B. A. M. (1993). Genetic manipulation of Campylobacter:
Evaluation of natural transformation and electro-transformation. Gene, 132(1), 131–135.
https://doi.org/10.1016/0378-1119(93)90525-8
Vegge, C., Brøndsted, L., Ligowska-Marzęta, M., & Ingmer, H. (2012). Natural Transformation of
Campylobacter jejuni Occurs Beyond Limits of Growth. Plos ONE, 7(9), e45467. doi:
10.1371/journal.pone.0045467
Miller, J. F., Dower, W. J., & Tompkins, L. S. (1988). High-voltage electroporation of bacteria: Genetic
transformation of Campylobacter jejuni with plasmid DNA. Proceedings of the National Academy of
Sciences, 85(3), 856–860. https://doi.org/10.1073/pnas.85.3.856
Beauchamp, J., Leveque, R., Dawid, S., & DiRita, V. (2017). Methylation-dependent DNA discrimination in
natural transformation ofCampylobacter jejuni. Proceedings Of The National Academy Of Sciences,
114(38), E8053-E8061. doi: 10.1073/pnas.1703331114
Van, T., Elshagmani, E., Gor, M., Scott, P., & Moore, R. (2016). Campylobacter hepaticus sp. nov., isolated
from chickens with spotty liver disease. International Journal Of Systematic And Evolutionary Microbiology,
66(11), 4518-4524. doi: 10.1099/ijsem.0.001383
Van, T., Gor, M.-C., Anwar, A., Scott, P., & Moore, R. (2017). Campylobacter hepaticus, the cause of spotty
liver disease in chickens, is present throughout the small intestine and caeca of infected birds. Veterinary
Microbiology, 207. https://doi.org/10.1016/j.vetmic.2017.06.022
Van, T. T. H., Lacey, J. A., Vezina, B., Phung, C., Anwar, A., Scott, P. C., & Moore, R. J. (2019). Survival
Mechanisms of Campylobacter hepaticus Identified by Genomic Analysis and Comparative Transcriptomic
Analysis of in vivo and in vitro Derived Bacteria. Frontiers in Microbiology, 10.
https://doi.org/10.3389/fmicb.2019.00107
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